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Re shown in Fig. 2. In the 72 h culture non-treated with IL-
Re shown in Fig. 2. In the 72 h culture non-treated with IL-1, the level of HA in G.4 was found to be increased 170 (p < 0.05) compared to G.1. After treatment of cells with IL-1, the level of GAG in G.4 compared to G.1 was significantly increased: 120 and 143 in 48 h and 72 h cultures, respectively. The level of HA also increased 400 and 600 in 48 h and 72 h cultures, respectively. The effect of siRNA on target mRNA expression The specificity of MMP-3 siRNA was determined by transfection of non-targeted siRNA as a control. Moreover, the selective suppression efficiency of MMP-3 siRNA wasassessed by analyzing the expression levels of other independent but functionally related transcripts (TIMP-3, HAS-1, HAS-2, AGG and COL2A1) in the same stages of all four groups. The results of this mRNA quantification show that MMP-3 siRNA triggered a remarkable suppression in the PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/10663624 amount of MMP-3 mRNA in the cells. As shown in Fig. 3, the relative expression level of MMP-3 mRNA in G.4 at 48 and 72 h was found to be reduced by 80 compared to G.1 (p 0.05) were observed in the relative abundance of these transcripts between the four groups. The relative expressions of all transcripts were compared between those treated and non-treated with IL-1 in the same group at the same time. We found almost all were different (p < 0.05), the relative expression of all genes being lower than in those non-treated with IL-1.DiscussionPro-inflammatory cytokines, such as IL-1 and other mediators produced by cytokine action on chondrocyte and synovial fibroblasts, may cause an imbalance in extracellular matrix (ECM) turnover, accelerate the degradation of the cartilage matrix, and also increase the incidence of chondrocyte apoptosis [18,19]. IL-1 appears to be first produced by the synovial membrane, and then diffuses into articular cartilage through the synovial fluid. It then activates chondrocytes, which in turnPage 5 of(page number PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/15901158 not for citation purposes)Journal of Orthopaedic Surgery and Research 2009, 4:http://www.josr-online.com/content/4/1/Figure (A), Viability 1 apoptosis (B) and mitotic (C) M617 rate in all experimental groups Viability (A), apoptosis (B) and mitotic (C) rate in all experimental groups. Individual bars show the mean ?SD. A significant difference (p < 0.05) between the four groups at the same condition (48 h non-IL1, 48 h IL1, 72 h non-IL1 and 72 h IL1) is displayed with superscript (a, b) on the bars. A significant difference (p < 0.05) between treatment and non-treatment with IL-1 at the same period (48 or 72 h) is displayed with superscript (*) on the bars. G1 = control group; G.2 = solution control; G.3 = non-silencing siRNA; and G.4 = MMP-3 siRNA.produce many catabolic factors. IL-1 has been implicated in the transcriptional upregulation of various MMPs, including MMP-1 [20,21] and MMP-3 [22,23]. For these reasons, this study used IL-1 as a typical inductor of an inflammatory metabolism. IL-1 was found to induce a distinct response in chondrosarcoma obtained from osteoarthritis chondrocytes. In this study, the viability and mitotic rate were shown to decrease, while the apoptosis rate increased significantly when treated with 10 ng/ml IL1. Moreover, prote.
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